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OBJECTIVE:To establish a method for simultaneous determination of 6 components in Niuhuang ninggong tablets.METHODS:HPLC method was adopted.The determination was performed on TC-C18 column with mobile phase consisted of methanol-0.05％ phosphate acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 280 nm,and the column temperature was 25 ℃.The sample size was 10 μL.RESULTS:The linear ranges of glycyrrhizin,berberine hydrochloride,baicalin,baicalin,emodin and chrysophanol were 3.2-320 μg/mL(r=0.999 9),8.8-880 μg/mL(r=0.999 8),5.6-560 μg/mL (r=0.999 5),2.0-200 μg/mL(r=0.999 9),4.4-440 μg/mL (r=0.999 9),2.0-200 μg/mL(r=0.999 7),respectively.RSDs of precision,stability and reproducibility tests were all lower than 6.0％.The recoveries were 96.34％-97.25％ (RSD=0.33％,n=6),96.12％-98.06％ (RSD=0.82％,n=6),96.36％-99.09％ (RSD=1.02％,n=6),95.84％-97.32％ (RSD=0.65％,n=6),95.83％-97.92％(RSD=0.88％,n=6),98.60％-99.65％(RSD=0.42％,n=6),respectively.CONCLUSIONS:The method is simple,accurate,stable and reproducible,and can be used for content determination of 6 components in Niuhuang ninggong tablets.
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OBJECTIVE:To establish a method for simultaneous determination of 6 components in Niuhuang ninggong tablets.METHODS:HPLC method was adopted.The determination was performed on TC-C18 column with mobile phase consisted of methanol-0.05％ phosphate acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 280 nm,and the column temperature was 25 ℃.The sample size was 10 μL.RESULTS:The linear ranges of glycyrrhizin,berberine hydrochloride,baicalin,baicalin,emodin and chrysophanol were 3.2-320 μg/mL(r=0.999 9),8.8-880 μg/mL(r=0.999 8),5.6-560 μg/mL (r=0.999 5),2.0-200 μg/mL(r=0.999 9),4.4-440 μg/mL (r=0.999 9),2.0-200 μg/mL(r=0.999 7),respectively.RSDs of precision,stability and reproducibility tests were all lower than 6.0％.The recoveries were 96.34％-97.25％ (RSD=0.33％,n=6),96.12％-98.06％ (RSD=0.82％,n=6),96.36％-99.09％ (RSD=1.02％,n=6),95.84％-97.32％ (RSD=0.65％,n=6),95.83％-97.92％(RSD=0.88％,n=6),98.60％-99.65％(RSD=0.42％,n=6),respectively.CONCLUSIONS:The method is simple,accurate,stable and reproducible,and can be used for content determination of 6 components in Niuhuang ninggong tablets.
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Objective To clone and analyze the coding region of methyltransferase gene from four species of Huperziaceae, the species included Huperzia serrata var.longipetiolata, Phlegariurus minchegensis, Phlegariurus austrosinicus and Phlegariurus petiolatus.Methods The methyltransferase coding region sequences were cloned by RT-PCR with the template of total RNA extracted from four Huperziaceae.Then the sequences were analyzed by means of BLAST and MEGA 5.0 software.ResuIts The coding region sequences of the four species of Huperziaceae were highly similar to the methyltransferase in the database.ConcIusion The methyltransferase genes of the four species of Huperziaceae were cloned in this study.The result will provide a foundation for further elucidate the mechanism of Huperzine A biosynthesis and metabolic pathways in Huperziaceae plants.
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Objective To observe the clinical efficacy and adverse reactions of meglumine in treartment of sudden deafness.Methods 60 pctients With sudden deafness were randomly divided into control group of 30 patients (conventional treatment)and the observation group(30 cases,meglumine treatment)before and after treatment,the ear hearing level changes in tirmitus,vertigo situation were compared.Results After a course of treatment,the observation group were cured,15 cases,10 cases,ineffective in 5 patients In the control group,10 cases markedly effective,effective in 5 cases,15 cases ineffective,clinical efficacy was significant(U =2.6 35,P ＜ 0.05).After treatment,hearing,tinnitus,dizziness and other symptoms significantly improved compared with before treatment(t =2.720,2.824,2.936,x2 =2.795,2.948,2.954,all P ＜ 0.05)after treatment,observation group improved more significantly than the control group(t =2.682,2.715,2.719,all P ＜ 0.05).Conclusion Meglumine in treatment of sudden deafness had exact effect satisfaction for the recovery of hearing.
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2 score,20.0% vs 8.5%) between two groups (P0.05). After a mean post-biopsy follow-up of (34.6?33.3) months, the 3-year and the 5-year renal survival rates for elder group were 74.6% and 62.2%, respectively, which were lower than those of non-elder group (100% and 92.9%, P=0.002). Conclusion:Elder patients with IgA nephropathy were more likely to suffer from hypertension, hyperlipidemia, renal insufficiency and chronic pathologic lesions, which might be the risk factors for the patient's unfavorable prognosis.
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0.05).In patients with HSPN capillary wall staining for IgA was more frequently found than in IgAN(71.0% vs 43.5%,P=0.013).With creatinine level doubling as the end point,the follow-up data indicated that the renal survival was 87.1% in HSPN and 91.9% in IgAN and there was no statistically significant difference between HSPN and IgAN(P=0.481).Conclusion:Although significant pathological difference was found between HSPN and IgAN,the renal clinical manifestations and long term outcome were similar between the two diseases in adults.